The study of Erigeron canadensis L. phenolic compounds
Keywords:
Erigeron canadensis L, HPLC, phenolic acids, flavonoids, validationAbstract
Analysis of the qualitative and quantitative composition of herb Erigeron canadensis L. phenolic compounds has been made by us using HPLC method. In water-spirit fraction obtained by extraction with heating, we have found 22 substances, 17 of them have been identified: phenolic acids, coumarins, flavonoids. The technique of the spectrophotometric determination of quantitative content of amount of flavonoids in the herb of Eri-geron canadensis L. has been developed and its validation has been carried out. We have established the following validation parameters of technique: precision (repeatability, reproducibility), accuracy and linearity. In determining linearity of the techniques we have established that the plots are linear. The correlation coefficient is close to one and it is equal to 0,9992 and 0,9991 for rutin and water-spirit extract of Erigeron canadensis L. herb respectively. In determining repeatability value of the relative standard deviation (RSD) was equal to 2,97 %. Acceptance criteria, expressed by value of RSD don’t exceed 3,84 %. As a result of the spent researches we have concluded that the technique is reproducible, affordable and express.References
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Published
2012-09-30
How to Cite
Ботов, А. Ю., В. Я. Яцюк, Г. В. Сипливый, and Л. Е. Сипливая. “The Study of Erigeron Canadensis L. Phenolic Compounds”. Traditional Medicine, no. 3(30) 2012, Sept. 2012, pp. 48-53, http://www.tradmed.ru/index.php/tm/article/view/316.
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Naturopathy
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