New approaches to standardization of herb of Hypericum

Authors

  • Р. Ш. Хазиев Kazan state medical university (Kazan, Russia)
  • Д. Н. Петрова Kazan state medical university (Kazan, Russia)
  • М. Н. Габтрахманова Kazan state medical university (Kazan, Russia)
  • А. Ю. Ситенков Kazan state medical university (Kazan, Russia)

Abstract

An express-method for quantitative determination of active substances in herbs of Hypericum perforatum and Hypericum maculatum, harvested in different areas of the Republic of Tatarstan, is developed. Optimal extraction conditions of flavonoids - principal active substances of these herbs, and conditions of their spectrophotometric determination after reaction with aluminum chloride have been determined. The accuracy of the method has been tested statistically, the error of the method does not exceed 0.9%. It is proposed to use for the analysis of the herb of H. maculatum the specific index of hyperoside (the dominant flavonoid of this plant) and aluminum chloride complex for calculations, and for the herb of H. perforatum - specific absorption index which takes into account the quantitative ratio of the two major flavonoids of this plant - hyperoside and routine.

Author Biographies

Р. Ш. Хазиев, Kazan state medical university (Kazan, Russia)

R.Sh. Khaziev

Д. Н. Петрова, Kazan state medical university (Kazan, Russia)

D.N. Petrova

М. Н. Габтрахманова, Kazan state medical university (Kazan, Russia)

M.N. Gabtrakhmanova

А. Ю. Ситенков, Kazan state medical university (Kazan, Russia)

A.Y. Sitenkov

References

1. Трава зверобоя: [фармакоп. ст.] // Государс-твенная Фармакопея СССР. - 11-е изд.: в 2 вып. / МЗ СССР. -Вып. 2: Лекарственное растительное сырье. - М.: 1989. - С.323.<BR>2. European Pharmacopoeia. - 5th ed. - Sup. 5.6. - Strasbourg: European Department for the Quality of Medicines, 2005.<BR>3. ФСП 42-7877-06 «Зверобоя трава» ОАО «Красногорсклексредства». - Введ. 18.09.2006. - М., 2006. - 14 с.<BR>4. Правдивцева О.Е., Куркин В.А. Исследование по обоснованию новых подходов к стандартизации сырья и препаратов зверобоя продырявленного // Химия растительного сырья. - 2008. - №1. - C.81-86.<BR>5. Зимина Л.Н., Куркин В.А., Рыжов В.М. Сравнительное исследование компонентного состава травы фармакопейных видов зверобоя методом высокоэффективной жидкостной хроматографии // Химия растительного сырья. - 2013. - №1. - C.205-208.<BR>
Традиционная медицина № 2 (41) 2015

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Published

2015-06-30

How to Cite

Хазиев, Р. Ш., Д. Н. Петрова, М. Н. Габтрахманова, and А. Ю. Ситенков. “New Approaches to Standardization of Herb of Hypericum”. Traditional Medicine, no. 2(41) 2015, June 2015, pp. 25-28, http://www.tradmed.ru/index.php/tm/article/view/449.

Issue

No. 2(41) 2015

Section

Naturopathy

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